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Synthego Inc chemically modified synthetic sgrnas
Chemically Modified Synthetic Sgrnas, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemically+modified+synthetic+sgrnas/chemically+modified+sgrna/10__7554_slash_elife__96285-311-3-10
Average 90 stars, based on 1 article reviews
chemically modified synthetic sgrnas - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Modification:

Article Title: Base-editing-mediated dissection of a γ-globin cis-regulatory element for the therapeutic reactivation of fetal hemoglobin expression.
Article Snippet: .. For RNAmediated base editing we used chemically modified synthetic sgRNAs harboring 2′-O-methyl analogs and 3′-phosphorothioate nonhydrolyzable linkages at the first three 5′ and 3′ nucleotides (Synthego). mRNA in vitro transcription In total, 10μg of BE-expressing plasmids were digested overnight with 20 Units of a restriction enzyme that cleaves once after the poly-A tail or after the stop codon, for constructs with or without a poly-A tail, respectively. .. The linearized plasmids were purified using a PCR purification kit (QIAGEN) and were eluted in 30μl of DNase/RNase-free water.

Article Title: A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS
Article Snippet: .. Chemically modified synthetic sgRNAs were purchased from Synthego (Menlo Park, CA, USA) and Cas9-NLS purified protein was from the QB3 MacroLab (UC Berkeley, CA, USA). ..

Article Title: Base-editing-mediated dissection of a γ-globin cis -regulatory element for the therapeutic reactivation of fetal hemoglobin expression
Article Snippet: .. For RNA-mediated base editing we used chemically modified synthetic sgRNAs harboring 2′-O-methyl analogs and 3′-phosphorothioate nonhydrolyzable linkages at the first three 5′ and 3′ nucleotides (Synthego). ..

Article Title: Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds
Article Snippet: Six-weeks-old female C57BL/6 mice were purchased from Janvier. .. For the gene knockout in THP1 cells using CRISPR/Cas9 technology, chemically modified synthetic sgRNAs were obtained from Synthego. ..

Article Title: VEXAS anemia is a mosaic erythroblastopenia
Article Snippet: .. We used chemically modified synthetic sgRNAs harboring 2′-O-methyl analogs and 3′-phosphorothioate nonhydrolyzable linkages at the first three 5′ and 3′ nucleotides (Synthego, Redwood City, CA). .. 2 × 10 5 to 1 x 10 6 HSPCs per condition were electroporated two days after thawing with 3.0 μg of the ABE-encoding mRNA and 3.2 μg of the synthetic sgRNA using the P3 primary cell 4D-Nucleofector X Kit S (Lonza, Basel, Switzerland) and the CA-137 program (Nucleofector 4D).

In Vitro:

Article Title: Base-editing-mediated dissection of a γ-globin cis-regulatory element for the therapeutic reactivation of fetal hemoglobin expression.
Article Snippet: .. For RNAmediated base editing we used chemically modified synthetic sgRNAs harboring 2′-O-methyl analogs and 3′-phosphorothioate nonhydrolyzable linkages at the first three 5′ and 3′ nucleotides (Synthego). mRNA in vitro transcription In total, 10μg of BE-expressing plasmids were digested overnight with 20 Units of a restriction enzyme that cleaves once after the poly-A tail or after the stop codon, for constructs with or without a poly-A tail, respectively. .. The linearized plasmids were purified using a PCR purification kit (QIAGEN) and were eluted in 30μl of DNase/RNase-free water.

Construct:

Article Title: Base-editing-mediated dissection of a γ-globin cis-regulatory element for the therapeutic reactivation of fetal hemoglobin expression.
Article Snippet: .. For RNAmediated base editing we used chemically modified synthetic sgRNAs harboring 2′-O-methyl analogs and 3′-phosphorothioate nonhydrolyzable linkages at the first three 5′ and 3′ nucleotides (Synthego). mRNA in vitro transcription In total, 10μg of BE-expressing plasmids were digested overnight with 20 Units of a restriction enzyme that cleaves once after the poly-A tail or after the stop codon, for constructs with or without a poly-A tail, respectively. .. The linearized plasmids were purified using a PCR purification kit (QIAGEN) and were eluted in 30μl of DNase/RNase-free water.

other:

Article Title: Atoh1 is required for the formation of lateral line electroreceptors and hair cells, whereas Foxg1 represses an electrosensory fate
Article Snippet: The sgRNAs were synthesized using the HiScribe T7 High Yield RNA 951 Synthesis Kit (NEB) and purified using the Monarch RNA Cleanup Kit (NEB) and stored at -952 80 °C before use.

Article Title: Correction of X-CGD patient HSPCs by targeted CYBB cDNA insertion using CRISPR/Cas9 with 53BP1 inhibition for enhanced homology-directed repair
Article Snippet: Chemically modified synthetic sgRNAs targeting sites in CYBB exon 1 or exon 2 were commercially synthesized (Synthego; Menlo Park, CA or TriLink Biotechnologies; San Diego, CA) with 2’-O-methyl 3’ phosphorothioate modifications at the first 3 and last 3 nucleotides for increased RNA stability and enhanced CRISPR/Cas9 editing activity [ ].

Purification:

Article Title: A single H/ACA small nucleolar RNA mediates tumor suppression downstream of oncogenic RAS
Article Snippet: .. Chemically modified synthetic sgRNAs were purchased from Synthego (Menlo Park, CA, USA) and Cas9-NLS purified protein was from the QB3 MacroLab (UC Berkeley, CA, USA). ..

Gene Knockout:

Article Title: Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds
Article Snippet: Six-weeks-old female C57BL/6 mice were purchased from Janvier. .. For the gene knockout in THP1 cells using CRISPR/Cas9 technology, chemically modified synthetic sgRNAs were obtained from Synthego. ..

CRISPR:

Article Title: Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds
Article Snippet: Six-weeks-old female C57BL/6 mice were purchased from Janvier. .. For the gene knockout in THP1 cells using CRISPR/Cas9 technology, chemically modified synthetic sgRNAs were obtained from Synthego. ..



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